Molecular biology techniques
The following table shows a very basic summary of molecular biology techniques
Enzyme-linked immunosorbent assay (ELISA)
| Technique | Description |
|---|---|
| Southern blotting | Detects DNA |
| Northern blotting | Detects RNA |
| Western blotting | Detects proteins Uses gel electrophoresis to separate native proteins by 3-D structure Examples include the confirmatory HIV test |
Molecular biology techniques
- SNOW (South - NOrth - West)
- DROP (DNA - RNA - Protein)
Enzyme-linked immunosorbent assay (ELISA)
- a type of biochemical assay used to detect antigens and antibodies
- a colour changing enzyme is attached to the antibody if looking for an antigen and to an antigen if looking for an antibody
- the sample therefore changes colour if the antigen or antibody is detected
- an example includes the initial HIV test
